ABSTRACT
The aim of present study was to investigate the antifungal activity of Hypericum perforatum extract in vitro. Three extraction techniques were used [chloroform, acetone and methanol solvents] and the extracts were tested against Fusarium chlamydosporum, Aspergillus niger, Aspergillus fumigatm, Aspergillus flavus. Acetone extract of H. perforatum was the most active against the growth of F. chlamydosporum, A. niger, it caused 55% and 40% inhibition of fungus growth, respectively, when used in a concentration of 7.5 mg/ml from growth media. The fungus A. flavus was more sensitive to the chloroform extract of H. perforatum and the inhibition percentage was 53.84% at the extract concentration of 7.5 mg/ml from growth media. The concentration 7.5 mg/ml of culture media for all extracts was more effective than the concentration 2.5 mg/ml from growth media. It was concluded that the acetone extract of H. perforatum showed a broad spectrum and greatest activity against the fungi among extracts tested